Leonardo Madrucciani
Advisor Petroleum LTD, UK
Title: Viral Detection by Nano-amplification Micro Array Technique
Biography
Biography: Leonardo Madrucciani
Abstract
Statement of the Problem: Microarray as a novel biological technique has been used in gene detection widely. But this method needs improvement, considering expensive probe labeling material and valuable signal scanner. So a visual detection technique was established by applying sandwich hybridization and silver stain. This technique was 100 times as sensitive as LCS (laser confocal scanning) and made it possible to develop a rapid and cheap detection method for Virus. Increasing studies revealed the application of gold nanoparticle as oligonucleotide label was wide.
Methodology & Theoretical Orientation
Materials of Viral detection microarray
Preparation of gold nano-particles described by Grabar (Grabar et al., 1995)
Preparation of detection microarray for Virus
Findings: Cumulative evidences showed gold labeled probe was efficient and could be alternative marker in microarray assay. On the other hand, the sliver stain enhancement, a technique companied with nano-gold labeling oligonucleotide, was used to amplify detection signal of nano-gold. This technique made detection signal visual and its sensitivity of gene detection was approximately 1000-fold high as that of Cy3-based fluorescence.